Microfluidics Devices for Next generation DNA Analysis

Objective:

To design and develop an integrated microfluidic platform for rapid, on-site DNA extraction, amplification, and detection.

Current Focus:

▪Performing isothermal amplification using  Recombinase   Polymerase Amplification (RPA)

▪ Optimising amplification parameters for maximum yield and sensitivity.

▪Integration of DNA extraction, amplification, and detection on a single microfluidic chip.

Expected Outcome:
A portable, low-cost microfluidic device enabling rapid, efficient, and user-friendly DNA analysis at the point of care.

Next Steps:

•Fabrication of microfluidic channels for controlled reaction flow.

•Embedding amplification zones to function using body temperature as a natural heat source.

•Validation of the integrated chip for real-time DNA amplification and detection.